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The purpose of this methodological guide is to outline how to conduct an empirical assessment of the current landscape of women’s small-scale fishery (SSF) organizations. Applying this methodology will enhance understanding of where women are organized in SSFs (both geographically and within the value chain); what their present organizational characteristics and capacities are; and their primary needs. Gathering this information through this methodology can help design interventions to enhance women’s organizational capacities and to work towards greater gender equality in fish value chains.